實時熱循環儀
Real Time Thermal Cycler NRTC-100 型號：NRTC-100
NRTC-100 是一種實驗室儀器，能夠執行可靠的分子生物學操作。它基於 Peltier 熱循環技術，可針對因不同溫度循環的偏差而導致的物理應力提供有效保護。配備菲涅耳透鏡，減少非目標區域的聚光，從而提高分析的靈敏度。
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- 獨特的外框保護設計
- 高效免維護 LED 光源
- 優化時間分辨信號分辨率和獨特的溫度控制技術
- 它在靈敏度、多色、串擾、溫度均勻性和準確性方面執行高級功能
- 支持所有類型的 QPCR 檢測模式
- 確保每個熒光通道的高重複性
- 以不同的間隔記錄同一樣品的不同熒光信號
- 高速步進電機和高靈敏度檢測器，確保在短時間內完成整個樣品板的所有信號採集
- 熔解曲線雙FAM掃描掃描時間大大縮短
- 有效降低邊緣溫度變化
- 它提供 USB 接口用於數據傳輸和與其他設備建立連接
- 軟體安裝可配置win10系統，適配QPCR各種流程，如參數設置功能、樣品信息記錄功能、檢測數據輸出功能、數據結果分析功能、故障提醒功能等
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樣品容量 0.1ml PCR管×96、8×12 PCR板或96孔板×1 反應體積 10-50 微升 最大加熱/冷卻速率 6℃/秒 加熱溫度範圍 4 °C - 100 °C 溫度精度 ±0.2°C 溫度均勻性 60°C 時 ±0.2°C，95°C 時 ±0.2°C 溫度梯度設置範圍 30°C-100°C 溫度梯度差設定範圍 1°C - 36°C 激發光源 4/6 單色高效 LED 探測器 光電倍增管 (PMT) 激發/檢測波長范圍 455-650 納米/510-715 納米 螢光通道數 4 支持染料 FAM/SYBR 綠、VIC/JOE/HEX/TET、ABY/NED/TAMRA/Cy3、JUN、ROX/德克薩斯紅、野馬紫、Cy5/LIZ 靈敏度 單拷貝基因 解析度 單重 qPCR 可區分 1.33 倍拷貝數差異 動態範圍 10個數量級的副本 外形尺寸 830×750×650毫米 淨重 22公斤 -
- Unique out frame protection design
- Efficient and maintenance free LED light source
- Optimized with time resolved signal resolution and unique temperature control technology
- It performs advanced level functionality with respect to sensitivity, multicolour, crosstalk, temperature uniformity and accuracy
- It supports all type of QPCR detection modes
- It ensures high repeatability of every single fluorescent channel
- Different fluorescent signals of the same sample are recorded at different intervals
- High speed stepping motor and highly sensitive detector ensures that all signal acquisition of the entire sample plate is completed in a short time duration
- Double FAM scanning of melting curve Scan time is shortened a lot
- Effectively reduces the edge temperature variance
- It provides USB interface for data transfer and to establish connectivity with other devices
- The software installation can be configured with win10 system to adapt QPCR for various processes like Parameter setting function, Sample information recording function, Detection data output function, Data result analysis function ,and Fault reminder function etc
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Sample capacity 0.1ml PCR tubes × 96, 8×12 PCR plate or 96 well plate ×1 Reaction volume 10-50 µl Maximum Heating/Cooling rate 6 °C/s Heating Temperature Range 4 °C - 100 °C Temperature Accuracy ±0.2°C Temperature Uniformity ±0.2°C at 60°C, ±0.2°C at 95°C Temperature gradients setting range 30°C-100°C Temperature gradient difference setting range 1°C - 36°C Excitation light source 4/6 monochrome high efficiency LEDs Detector Photomultiplier tube (PMT) Excitation/detection wavelength range 455-650 nm/510-715 nm No. of Fluorescent channels 4 Supported dye FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/ Texas Red, Mustang Purple, Cy5/LIZ Sensitivity Single copy gene Resolution 1.33 folds copy number difference can be distinguished in single-plex qPCR Dynamic range 10 orders of magnitude copies Overall dimensions 830×750×650 mm Net Weight 22 kg